Program – Winter School 2023

Please consider time for discussion for your talk. Standard talks are scheduled for 12 min, i.e. 8 min presentation plus 4 min discussion

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Wednesday, March 1

18:00 DINNER (Paradies, Laurin)

20:00 – 22:00   Wednesday evening session: Alzheimer’s Disease & Quality Control

  • 20:00 – 20:10 Opening: Klaudia BRIX & Hans BRANDSTETTER
  • 20:10 – 20:20 Chairs: Christoph BECKER-PAULY, Marcin DRAG & Kathrin GNOTH
  • 20:20     BICKENBACH Kira (Christian-Albrechts University Kiel)  Meprin β is a regulator of blood-brain barrier integrity
  • 20:32     ARMBRUST Fred (Christian-Albrechts University Kiel)  Meprin β is an alternative β-secretase and associated with the development of Alzheimer’s disease
  • 20:45     HOLZNER Christoph (Paris-Lodron-Universität Salzburg)  Legumain may function as a transient TrkB sheddase
  • 20:57     OSOBLE Nimo Mukhtar Mohamud (University of Oslo)  Involvement of legumain in differentiation and energy metabolism in human myotubes
  • 21:10     GARCIA Victoria (University Clinic Freiburg)  Enzymatic and non-enzymatic functions of the SUMO isopeptidase USPL1 in vivo
  • 21:22     ŹMUDZIŃSKI Mikołaj (Wrocław University of Science and Technology)  C-terminal modified ubiquitin derivatives as novel selective activity-based probes for  deubiquitinating enzymes                
  • 21:35     KNOBELOCH Klaus-Peter (University Freiburg)  Enzymatic and non-enzymatic functions of Ubiquitin-specific protease 18

22:00  HANDS-ON SESSION ON PROTEASES AND HYDROLASES. AN INTRODUCTION (PARADIES).

Thursday, March 2

09:00 – 10:50   Thursday morning session I: Organ-specific Proteases – Skin & Kidney

  • 09:00 – 09:10  Chairs: Yoshi ITOH, Marcin POREBA, Walter STÖCKER
  • 09:10     CHRISTIANSEN Chatpakorn Rassameena (Technical University of Denmark) Target discovery in Netherton Syndrome Organotypic Model by N-TAILS
  • 09:22     CANBAY Vahap (Technical University of Denmark) Functional characterization of dermokine in epidermal differentiation
  • 09:35     DOHNALEK Jan (Institute of Organic Chemistry and Biochemistry) Rhomboid protease RHBDL2 is a calcium activated suppressor of EGFR signaling in epithelial cells
  • 09:47     GUDAITIS Simonas (Christian-Albrechts University Kiel) β-dystroglycan: analysis of a novel ADAM10 substrate and its suspected role in podocytopathies
  • 10:00     KÜHNE Stine (Christian-Albrechts University Kiel) Analysis of the suspected role of tetraspanin 15/5 in the regulation of ADAM10 and in podocyte injury
  • 10:12     RICKMAN Olivia (University of Lausanne) Transmembrane serine protease 2 (TMPRSS2) regulates the α subunit of the epithelial sodium channel (ENaC)
  • 10:25     JOOS Corentin (Christian-Albrechts-University Kiel) Functional role of meprin metalloproteases in the kidney
  • 10:37     FORBORD Karl Martin (University of Oslo) The interplay between the cysteine protease legumain and vitamin D in vivo

10:50 – 11:20   COFFEE BREAK

11:20 – 12:22   Thursday morning session II: Thyroid-specific proteases & Cancer detection methods

  • 11:20     DOGRU Gaye Alara (Constructor University)  Investigation on primary cilia of Nthy-ori 3-1 cells upon cysteine cathepsin inhibition or thyrotropin stimulation.
  • 11:32     MÜLLER Janina (Constructor University)  Influence of Thyroid Hormone Receptor Signaling on Thyrocyte Auto-Regulation
  • 11:45     BUEMI Frederic (Imperial College London)  Detecting hydrolytic enzymes driving colorectal cancer development
  • 11:57     CWILICHOWSKA Natalia (Wrocław University of Science and Technology) Dissecting proteolytic pattern in breast cancer patients using mass cytometry
  • 12:10     MAJCHRZAK Martyna (Wrocław University of Science and Technology) Targeting cancer with selective prodrugs activated by prolyl proteases

12:30   LUNCH (Paradies, Laurin)

14:30 – 15:42   Thursday afternoon session I: Cancer mechanisms

  • 14:30 – 14:40 Chairs: Ruth GEISS-FRIEDLANDER, Alejandro GOMEZ-AULI, & Christoph PETERS
  • 14:40     BHAT Mahima (Universität Freiburg)  Combinatorial targeting of the PI3K oncogenic signaling pathway and methionine aminopeptidases in breast cancer
  • 14:52     DAVID Nele (Christian-Albrechts-University Kiel)  The cancer-associated meprin β-variant R238Q exhibits altered cleavage specificity and impacts glioma cell migration
  • 15:05     STEIDLE Malina (Christian-Albrechts University Kiel)  Characterization of the proteolytic properties of cancer-associated variants of meprin β
  • 15:17     DREYER Tobias (TU Munich)  Kallikrein-related peptidase 7 (KLK7) mediates immune escape and olaparib resistance in BRCA2-deficient ovarian cancer
  • 15:30     HESS Lisa (Universität Freiburg)  Dipeptidyl-peptidase 9 regulates the dynamics of tumorigenesis and metastasis in breast cancer

15:42 – 16:10   COFFEE BREAK

16:10 – 18:00   Thursday afternoon session II: Discovering substrates and their proteases

  • 16:10 – 16:20 Chairs: Ulrich AUF DEM KELLER, Chris OVERALL & Boris TURK
  • 16:20     KALOGEROPOULOS Konstantinos (Technical University of Denmark)  CLIPPER 2.0: Peptide level annotation and data analysis for positional proteomics
  • 16:32     KOLARIČ Matej (Jožef Stefan Institute)  PeptideVisualizer: an open-source cross-platform software solution for PROTOMAP-based determination of protease substrates
  • 16:45     HAACK Moldt Aleksander (Technical University of Denmark)  Fast Automated N-Terminome Analysis (FANTA) – Single day automated mass spectrometry based degradomics
  • 16:57     MODRZYCKA Sylwia (Wrocław University of Science and Technology) Parallel imaging of coagulation pathway proteases in human plasma
  • 17:10     WEISS Konstantin (Universität Köln)  Exploring activity regulation of DPP9 using a genetically encoded activity sensor
  • 17:22     DONZELLI Laura (Albert-Ludwigs-University Freiburg)  The role of DPP9 in endoplasmic reticulum stress
  • 17:35     WIRTGEN Valentina (University of Freiburg)  The Interactome of DPP9 under ER stress
  • 17:47     ZOLG Samuel (Institute of Molecular Medicine and Cell Research)  Analysis and characterization of novel dipeptidyl peptidase 9 substrates under DNA damage conditions
  • 18:00     BRIX Klaudia (Constructor University) The ‘ProteoCure’ COST Action

18:30   DINNER (Paradies, Laurin)

20:00 – 23:00   Intense training in proteolytic assays: Quantitative assessments and quality control (PARADIES)

Friday, March 3

09:00 – 10:50   Friday morning session I: Proteolysis in Virology

  • 09:00 – 09:10 Chairs: Charaf BENARAFA, Paulina KASPERKIEWICZ & Robert VIDMAR
  • 09:10     LEBORGNE Nathan (Institute of Virology and Immunology)  Neutrophil serine proteases elastase and cathepsin G cleave SARS-CoV-2 spike protein and dampen virus-mediated inflammation
  • 09:22     GRIN Peter (University of British Colombia)  Defeating an antiviral interferon response by SARS-CoV-2 main protease (3CLpro) cleavage
  • 09:35     NARWAL Manju (Pennsylvania State University)  SARS-CoV-2 polyprotein substrate regulates the stepwise Mpro cleavage reaction
  • 09:47     VOGELE Daniel canceled (Universitätsklinikum Freiburg)
  • 10:00     OLIVEIRA Bernardo Reinaux Silva (Constructor University)  Cytoskeletal changes in neuroblastoma cells upon trimeric Spike protein treatment
  • 10:12     BAJABA Shyline Tariro (Constructor University)  Incubation with SARS-CoV-2 Spike-Protein Affects Neuronal Microtubules by Altering Tau Proteins in Glial Cell Co-Culture Models
  • 10:25     HENSCHEL Maike (Constructor University)  Incubation with SARS-CoV-2 Spike-Protein influences the Intestinal Barrier Integrity in an Organ-typical Co-culture Model
  • 10:37     SCHMIDT Dorothea (Constructor University)  The effect of dietary polyphenols on mucus production by intestinal epithelial cells

10:50 – 11:15   COFFEE BREAK

11:15 – 12:15   Friday morning session II: Bacteria

  • 11:15 – 11:25 Chairs: Ulrich ECKHARD, Xavier GOMIS-RÜTH & Jan POTEMPA
  • 11:25     CHARL Julia (University of Cologne)  Conformational changes in the asymmetric ATPase ring of the metalloprotease FtsH
  • 11:37     VAN BELJOUW Sam (Delft University of Technology)  Craspase is a CRISPR RNA-guided, RNA-activated protease
  • 11:50     HOI David (Institute of Molecular Pathology)  Induced self-elimination of the Clp protease system as antibiotics strategy
  • 12:02     LOVE Liam (Queensland University of Technology)  Recombinant expression of the Chlamydial Protease-like Activity Factor (CPAF) from Chlamydia trachomatis

12:30   LUNCH (Paradies, Laurin)

14:30 – 17:45   Friday afternoon session: Inhibitors & mechanisms

  • 14:30 – 14:40 Chairs: Jonathan HARRIS & Markus KAISER
  • 14:40     DUFF Alex (Queensland University of Technology)  Designing clinically relevant inhibitors of KLK8: a structure and function-based approach
  • 14:52     FEHR Lorenz (Universität Duisburg-Essen)  Dipeptidyl Peptidase 8 and 9 Inhibitors Based on the Natural Product Sulphostin
  • 15:05     TABAK Werner canceled (Universität Duisburg-Essen)
  • 15:17     DAHMS Sven (Paris-Lodron-University Salzburg)  Induced-fit binding of dichlorophenylpyridine-based furin inhibitors
  • 15:30     STEINMETZER Torsten (Philipps University Marburg)  Structure-based design, synthesis, and characterization of macrocyclic serine protease inhibitors

15:50 – 16:20   COFFEE BREAK

  • 16:20     GRANT Cameron (Queensland University of Technology)  Inhibition of Human Neutrophil Elastase by the Microbial Inhibitor, Ecotin is Abrogated by Neutrophil Oxidation
  • 16:32     JACKSON Alex (Queensland University of Technology)  Design of a dual-domain human-derived anti-fibrinolytic and anti-inflammatory protease inhibitor
  • 16:45     KORKMAZ Brice (University of Tours)  The past, present and future of pharmacological targeting of cathepsin C
  • 16:57     KIENZL Nikolaus (BOKU Vienna)  Human cathepsin O: a lysosomal cysteine endopeptidase that cannot autoprocess
  • 17:10     SHAFIEI Roya (Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)) Phosphonate inhibitors targeting Pseudomonas aeruginosa elastase LasB demonstrate promising activity in both in vitro and in vivo studies
  • 17:22     KLEIN Andreas (Helmholtz Institute for Pharmaceutical Research Saarland (HIPS)) LasB inhibitor characterization by novel lung homogenate based assays and X-ray crystallography
  • 17:35     REINHECKEL Thomas (University of Freiburg)  Do or Die: An Intro to Tiers Extreme Hiking

18:00   DINNER (Paradies, Laurin)

19:00 – 20:00   Sample preparation in protease research (CITY HALL)

20:15 – 23:45   Acoustics and kinetics in enzymology, featuring InhibiTIERS (CITY HALL)

inhibitiers
© Viktor Magdolen

Saturday, March 4

09:00 – 11:45   Saturday morning session: Immunity & Inflammation

  • 09:00 – 09:10 Chairs: Achim KRÜGER & Jennifer VANDOOREN
  • 09:10     RAHN Sascha (Christian-Albrechts University Kiel)  Insights into pathological meprin α activity as a molecular driver and novel biomarker of systemic inflammation
  • 09:22     DOMAIN Roxane (University of Tours)  Proteolytic pathways involved in the activation of pro-inflammatory neutrophil serine proteases
  • 09:35     KRAUZE Izabela (Wrocław University of Science and Technology)  Differences in expression and activities of NSPs in neutrophils in health and diseases
  • 09:47     KORBA Aleksandra (Wrocław University of Science and Technology)  Function of granzyme A in cell death of neutrophils
  • 10:00     HÄUSSLER Daniel (TU Munich) The emerging proinflammatory cytokine TIMP-1: Combination of in-silico docking & wet-lab experiments reveal the functional binding of TIMP-1 with CD74
  • 10:12     FRÄDRICH Julian (TU Munich)  The emerging pro-inflammatory cytokine TIMP-1: Identification of TIMP-1 as a functional ligand of Amyloid Precursor Protein in monocytes

10:25 – 10:50   COFFEE BREAK

  • 10:50     BERNAERTS Eline (KU Leuven)  Macrophages shift subcellular metalloprotease activity depending on their activation state
  • 11:02     CALLIGARIS Matteo (University of Pisa)  High-resolution shotgun proteomic reveals a novel function of inactive rhomboids 2 in major histocompatibility complex 1 surface expression and       CD8+ T cell activity
  • 11:15     PARUI Ishita (Universitätsklinikum Freiburg)  Caspases can regulate cytokine secretion during sub-lethal apoptosis signalling
  • 11:27     CROIS Anna (University of Freiburg)  Non-Apoptotic signalling pathways involving caspase-8
  • 11:40     Hans BRANDSTETTER (Paris-Lodron-University of Salzburg) Closing remarks & end of scientific sessions

12:00   LUNCH (Paradies, Laurin)

SATURDAY AFTERNOON: HIKING, EXCURSION, ROUND TABLE DISCUSSIONS

  • 12:15 – 17:00     “Extreme” hiking tour (lunch package will be provided)
  • 12:15 – 17:00     Locally organized skiing tours (ski equipment can be rent at the Hotel Paradies; lunch package will be provided)
  • 12:45 – 16:00     Bus tour to Bozen (Guided Tour of Bozen, Visit Ötzi & more)
  • 17:45                Shuttle bus to Castle Haselburg (Bozen)

19:00   GALA DINNER AT CASTLE HASELBURG

Haselburg3
© Castle Haselburg

Sunday, March 5

10:00     Bus departure

Abstracts for oral presentations are invited for research on proteases in their molecular, cellular and organismic context. Methodological advances for studying aspects of the complex protease web as well as their interaction with substrates, activators and inhibitors are similarly highly sought after. When preparing your abstract, please follow the guidelines given under “Registration & Abstract Submission”.