Winter School 2022

The online sessions will take place 3pm – 9pm CET each day (EST: – 6 hours, Pacific Standard Time: – 9 hours)

 

Wednesday, 16 February

Welcome     15:00-15:05     Klaudia Brix, Hans Brandstetter

  • Session 1 – Cancer, KLKs
    • 15:05 -15:15   Chairs: Tobias Dreyer, Barbara Grünwald   Introduction
    • 15:15 -15:30   Isabel VOGEL   Kallikrein-related peptidase-mediated gene regulation of keratin 19 and moesin as a potential therapy resistance mechanism in high-grade serous ovarian cancer
    • 15:30 -15:45   Giulia MAGNO   Kallikrein-related peptidase KLK7 as a new target to improve immunotherapy in ovarian cancer
    • 15:45 -16:00   Thien-Ly Julia DINH   Proteomic analysis of non-muscle and muscle-invasive bladder cancer highlights distinct subgroups
    • 16:00 -16:15   Maren Nicole STILLGER   Calpain-2 is Overexpressed in Glioblastoma multiforme and Contributes to Temozolomide Resistance
    • 16:15 -16:30   Daria ARBUZNIKOVA   Modulation of PSMA Expression Levels on Prostate Cancer Cells by External Beam Irradiation
    • 16:30 -16:45   Natasha ALEY   ATG4B mediates beta-catenin expression and 3D spheroid formation in pancreatic ductal adenocarcinoma
  • Session 2 – DPP8/9
    • 17:15 -17:30   Chairs: Ruth Geiss-Friedlander, Andreas Bachmair, Markus Kaiser   Introduction
    • 17:30 -17:45   Lisa HESS   Stage-dependent functions of dipeptidyl peptidase 9 in mammary cancer progression
    • 17:45 -18:00   Laura DONZELLI   The role of DPP9 in the unfolded protein response and ATF6 signalling
    • 18:00 -18:15   Svenja WÖHRLE   Inflammasome Activation by DPP8/9 inhibitors
    • 18:15 -18:30   Lorenz FEHR   Development of DPP8/9 Inhibitors
  • Session 3 – Caspases & lysosomal proteases in health and disease
    • 19:30 -19:45   Chairs: Elfriede Dall, Simon Stael, Gilles Lalmanach   Introduction
    • 19:45 -20:00   Ishita PARUI   Caspase mediated proteolysis in lethal and sub-lethal apoptosis signalling
    • 20:00 -20:15   Małgorzata KALINKA   Selective chemical reagents to investigate the role of caspase 6 in apoptosis in acute leukemia T cells
    • 20:15 -20:30   Oliwia GORZEŃ   New generation of anti-cancer prodrugs activated by lysosomal proteases
    • 20:30 -20:45   Tasneem ELAMIN   Regulatory aspects of legumain activity and its inhibition by cystatin E
    • 20:45 -21:00   Lisa GALLWITZ   Cathepsin D´s role in neurodegeneration and as a therapeutic drug in conditions of neurodegeneration

Thursday, 17 February

  • Session 1 – ECM remodelling & hemostasis
    • 15:00 -15:15   Chairs: Christoph Becker-Pauly, Ulrich auf dem Keller, Chris Overall   Introduction
    • 15:15 -15:30   Zuzanna Helena BALD   A mutation in ST14 that can provide insight into the stability of matriptase-HAI complexes
    • 15:30 -15:45   Konstantinos KALOGEROPOULOS   Proteomic and proteolytic landscape of acute human skin repair
    • 15:45 -16:00   Ruben Siedlarczyk NOGUEIRA   Recombinant plant plasma Kallikrein inhibitor delays artery occlusion and thrombus size with no bleeding
    • 16:00 -16:15   Elisabeth NYSTRÖM   Colonic mucus proteolysis in colitis
    • 16:15 -16:30   Annika W. NONBOE   Deficient prostasin activation by matriptase mutants may cause Ichthyosis-Hypotrichosis Syndrome
    • 16:30 -16:45   –
  • Session 2 – Pathogens
    • 17:15 -17:30   Chairs: Ulrich Eckhard, Evelyn Hutterer, Walter Stöcker   Introduction
    • 17:30 -17:45   Katarzyna MIKRUTA   Study of the interaction between RagAB peptide transporter and TonB from Porphyromonas gingivalis
    • 17:45 -18:00   Anna JACULA   Hemagglutinin/adhesin domains play a crucial role in maturation of gingipains
    • 18:00 -18:15   Jamil SERWANJA   Chew and Digest Mechanism of Bacterial Collagenolysis by Conformational control of the activator and peptidase domains of the Collagenase Unit
    • 18:15 -18:30   Alaa ALHAYEK   Discovery and Characterization of Novel, Potent Inhibitors of Clostridial and Bacillus Collagenases
  • Session 3 – Poster session (19:30 – 21:00)
    • Chairs: Johannes Eble, Yoshi Itoh
    • Natalia ĆWILIKOWSKA   Detection of tumor markers in breast cancer using mass cytometry
    • Oguz BOLGI   DPP9 plays a role in Homologous recombination and regulates BRCA2 stability via the N-Degron
    • Claire STARK   KLK7 as modulator of PARP inhibition

       

    • Tobias DREYER   KLK4 induced immune escape in ovarian cancer
    • Roxane DOMAIN   Cathepsin C-independent maturation of granzyme zymogens in cytotoxic lymphocytes

       

    • Vahap CANBAY   Functional characterization of dermokine in epidermal differentiation
    • Christoph HOLZNER   Quality Control of Neurtrophin Production

       

    • Daniel VOGELE   Substrate Selectivity of SARS-CoV-2 papain-like Protease Plpro
    • Naiá Porã SANTOS   Functional analysis of phytocystatin 6 from Arabidopsis thaliana revealed amyloid fibril formation
    • Miguel COSENZA-CONTRERAS   FragNterminomics: a data analysis approach to extract information related to proteolytic activity from shotgun proteomics data

Friday, 18 February

  • Session 1 – Protease engineering & Inhibitor design, method development
    • 15:00 -15:15   Chairs: Pitter Huesgen, Evette Radisky, Christian Sommerhoff   Introduction
    • 15:15 -15:30   Matt COBAN   Selectively targeting mesotrypsin via a novel allosteric inhibitory ligand
    • 15:30 -15:45   Tanmaye Nallan CHAKRAVARTHULA     Comparison of Plasmin Inhibition with Monovalent and Bivalent Inhibitors 
    • 15:45 -16:00   Eran MAINA   Computational Investigation of enhanced MMP binding by an engineered TIMP1 variant
    • 16:00 -16:15   Bibo Betsaida VERDUGO   Resurrection of an ancient inflammatory locus reveals switch to caspase-1 specificity on a caspase-4 scaffold
    • 16:15 -16:30   Rawad HANNA   LysN Amino Terminal Enrichment -LATE- a new method for N-terminal enrichment
    • 16:30 -16:45   Soumili SARKAR   Protease-based tailoring of 3D hepatic organoids
  • Session 2 – Virus
    • 17:15 -17:30   Chairs: Chris Scott, Roberta Burden, Rich Williams   Introduction
    • 17:30 -17:45   Sanjay SAW   Competitive advantage of TIMP2 in hematopoietic stem cell during bone marrow regeneration
    • 17:45 -18:00   Emma CLYDE   The development of an inhaled Mpro inhibitor for the treatment of respiratory viral infections
    • 18:00 -18:15   Ryan JEEP     Catalytic Flexibility Revealed through HIV Protease Drug Resistance Analyses by Various Assays
    • 18:15 -18:30   James HARTE   Regulated Intramembrane Proteolysis of ACE2: A Proposed Role of Host Factor Cleavage in SARS-CoV-2 Syncytiation
    • 18:30 -18:50   Chris OVERALL   Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome
  • Session 3 – Award ceremony, Frizzies; birthdays RH & WoBo (19:30 – 21:00)

Frizzie

Abstracts for oral presentations are invited for research on proteases in their molecular, cellular and organismic context. Methodological advances for studying aspects of the complex protease web as well as their interaction with substrates, activators and inhibitors are similarly highly sought after. When preparing your abstract, please follow the guidelines given under “Registration & Abstract Submission”.