Christoph Szymoniuk ist Gebietsleiter Cell Pathology bei A. Menarini GmbH in Wien.
Abstract:
DEPArray™ is the only sorting technology capable of isolating, through a semiconductor controlled microfluidic chip, 100% pure single or pooled cells. Single Circulating Tumor Cells can be isolated from enriched blood, pools of pure Tumor, EMT, TILs and Stromal cells can be isolated  from disaggregated FFPE tissue blocks as well as live cells can be isolated from fresh tumor tissue. Combined with the FDA approved Cellsearch® workflow for counting tumor cells from blood samples, Menarini Silicon Biosystems offers a complete and unique Liquid Biopsy workflow NGS Results show how interpretation of the underlying genetic alterations becomes a lot clearer on samples transformed into collections of pure or single cells, not only improving the detection of Somatic Mutations but enabling highly informative Copy Number Aberration analysis  on the pure cell populations. During the seminar we will present the principles of operation of the technology, testing and validation of the accuracy of the method with NGS panels and Whole Exome Sequencing, applications in the area of single CTC genetic analysis and FFPE tumor tissues.