Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579
Carmen M. Abfalter, Esther Schönauer, Karthe Ponnuraj, Markus Huemer, Gabriele Gadermaier, Christof Regl, Peter Briza, Fatima Ferreira, Christian G. Huber, Hans Brandstetter,
Gernot Posselt, Silja Wessler
Abstract:
Bacterial collagenases differ considerably in their structure and functions. The collagenases ColH and ColG from Clostridium histolyticum and ColA expressed by Clostridium perfringens are well-characterized collagenases that cleave triple-helical collagen, which were therefore termed as ´true´ collagenases. ColA from Bacillus cereus (B. cereus) has been added to the collection of true collagenases. However, the molecular characteristics of B. cereus ColA are less understood. In this study, we identified ColA as a secreted true collagenase from B.cereus ATCC 14579, which is transcriptionally controlled by the regulon phospholipase C regulator (PlcR).
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Reviewed by Gerhard Obermeyer