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Protists from the 3D-printer, 1st June 2022

The recently opened exhibition of the Haus der Natur displays two new models of protists at 5,000 to 6,000× magnification ( https://www.hausdernatur.at/de/). The models of a ciliate (Ciliophora) of the genus Euplotes and of a testate amoeba (Cercozoa) of the genus Euglypha were created in close collaboration with the research group Protistology of Assoc. Prof. Dr. Sabine Agatha. The 3D models have prominent position in the showcase “Biodiversity” and demonstrate impressively the complexity of unicellular organisms. Actually, these single cells are consummate organisms that perform locomotion, feed, digest, defecate, proliferate, and even have sex. The numerous genders of ciliates necessitate a particularly elaborate mate selection by means of pheromones.

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Lange Nacht der Forschung (Long Night of Science), 20th May 2022

The “Long Night of Science” at the Faculty of Catholic Theology and in the assembly hall of the University of Salzburg was extremely successful. The research group Protistology presented many visitors a huge variety of microscopic organisms living in freshwater, mainly unicellular organisms (protists) with bizarre lifestyles (Courses).

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City Nature Challenge, April 2022

The research group Protistology and several interested students microscopically studied and identified numerous protists and mesozooplankton organisms from puddles, ponds, and lake in Salzburg and added them to the records of the City Nature Challenge. Finally, Salzburg compiled 12.653 observations including microscopic organisms. Thereby, Salzburg became second best in Austria and Europe and was among the top 5% worldwide.

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Cover picture of scientific journal, January 2022

The 3D reconstruction of the somatic infraciliature by Michael Gruber, former PhD student in the research group Protistology, is cover picture of the international peer-reviewed Journal of Eukaryotic Microbiology published by Wiley.Foto


Sabine Agatha among the World’s top 2 per cent of Scientists

The team of John P. A. Ioannidis from the Stanford University recently published a ranking of the world’s top 2% of scientists (Ioannidis JPA, Boyack KW, Baas J (2020) Updated science-wide author databases of standardized citation indicators. PLoS Biol 18(10): e3000918.  https://doi.org/10.1371/journal.pbio.3000918). They assessed the citation impact during the calendar year 2019 (Table-S7-singleyr-2019) and listed S. Agatha among the top 2%.


Publication of the Month – May 2020 for Maximilian Ganser and Thomas Hütter

“DeSignate: detecting signature characters in gene sequence alignments for taxon diagnoses”Maximilian H Ganser, Thomas Hütter, Manuel Kocher, Merima Halkic, Sabine Agatha & Nikolaus Augsten

Abstract:  Species of prokaryotes and eukaryotes including plants, animals, fungi, and unicellular protists are described and named by taxonomists. These descriptions include species diagnoses which state all characters distinguishing a species from its closest known relatives. Diagnostic characters mainly comprise morphological differences while molecular differences are rarely included because efficient solutions are lacking. We therefore developed the software tool DeSignate in an interdisciplinary project between the Protistology Research Group (AG Agatha, Biosciences) and the Database Research Group (AG Augsten, Computer Sciences). DeSignate enables the detection and classification of diagnostic molecular characters in a user-friendly web interface which can subsequently be included in diagnoses. For the program see,  https://designate.dbresearch.uni-salzburg.at/.

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Introduction into Electron Microscopy for Pupils from Grammar School Zaunergasse

Two groups of pupils from the grammar school in the Zaunergasse in Salzburg visited together with their teacher Leopoldine Sams the Department of Biosciences on February 18th and 19th. Prof. Ulrike Berninger gave a short overview of the biological studies at the University of Salzburg. Then, Assoc.-Prof. Dr. Sabine Agatha and Mag. Birgit Weissenbacher from the working group ‘Protistology’ gave an introduction into scanning and transmission electron microscopy. The functioning of the electron microscopes and the time-consuming preparation of the samples, using sophisticated techniques and facilities, was demonstrated in own videos. A tour through the department with a detailed demonstration of the electron microscopical facilities completed the visit. All participants, the teacher, and the staff of the Department of Biosciences had a lot of fun.

Pupils of grammar school Zaunergasse


New Videos

Videos on preparations for scanning and transmission electron microscopy of protists as well as on live observation of the model ciliate Paramecium sp. were produced by the AG Agatha; they are available under “Lehre“.


Poster price for Michael Gruber at Meeting of NOBIS Austria

At the 13th annual meeting (November 28th & 29th 2019) of the Network of Biological Systematics (NOBIS) Austria in Salzburg, the poster of Michael Gruber with the title “Tintinnid’s first top model: ultrastructural insights into the oral ciliature of Schmidingerella meunieri (Alveolata, Ciliophora)” was rewarded.

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Awardees: Susanne Affenzeller (best talk of a PhD student), Michael Gruber (AG Agatha; best poster), and Silvester Bartsch (best talk of a Master student).


With three talks and four posters at the European Congress of Protistology in Rome

The European Congress of Protistology takes place every four years and participants come not only from European countries, but also from North and South America, China, Japan, Korea, and India to name only the most dominant ones. This year, the congress took place in Rome, Italy, from July 28th to August 2nd. Sabine Agatha, Maximilian Ganser, and Michael Gruber presented their recent results in three talks (incl. one plenary talk) and three posters covering several mainly ultrastructural aspects of tintinnid ciliates, viz., the oral and somatic ciliature, the resting cyst, the extrusomes (capsules), and the lorica walls. Additionally, the findings on the diversity of tintinnids from the Northeast Pacific including supposedly new species was shown.


Our article is one of the top downloaded!

Our article “Redescription of Tintinnopsis everta Kofoid and Campbell 1929 (Alveolata, Ciliophora, Tintinnina) Based on Taxonomic and Genetic Analyses-Discovery of a New Complex Ciliary Pattern”, published in the Journal of Eukaryotic Microbiology (65: 484–504; doi: 10.1111/jeu.12496), is one of the journal’s top downloaded recent papers! This means: Amongst articles published between January 2017 and December 2018, our paper received some of the most downloads in the 12 months following online publication. Thus, our work generated immediate impact and visibility, contributing significantly to the advancement of your field.


“Microscopic Life in Soil” Unterrichtseinheiten für die 6. Klasse AHS Oberstufe konzipiert von Frau Mag. rer. nat. Susanne Riedl

Protozoen beeinflussen die Bodenfruchtbarkeit und somit die Nahrungsverfügbarkeit für den Menschen, indirekt über den Einfluss auf die Bakterienpopulation, die Hauptzersetzer der Streu, und direkt durch die Abgaben von Stickstoffverbindungen. Im Gegensatz zu Schulbüchern, welche sich üblicherweise auf Metazoen fokussieren und dabei die Protozoen außer Acht lassen, führt die vorliegende Diplomarbeit Unterrichtseinheiten ein, welche sich auf die spezifischen Anpassungen an den ephemeren Charakter des Bodens sowohl der Protozoen als auch der Metazoen konzentrieren und einen vereinfachten Einblick in das Bodennahrungsnetz liefern. Trotz ihrer mikroskopischen Größe sind Protozoen, insbesondere Ciliaten, einfach zu handhaben und liefern genügend morphologische Kennzeichen zur Unterscheidung der Hauptgruppen. Dementsprechend ist es für die Schülerinnen und Schüler möglich, ihre eigenen Hypothesen in den praktischen Einheiten bezüglich der Sukzession, des Nahrungsnetzes, dem Einfluss von Streu und der Wasserverfügbarkeit auf die Abundanz der Protozoen im Boden zu prüfen. Durch die Abdeckung dieser Themen fügen sich die Einheiten nicht nur perfekt in den Lehrplan der österreichischen höheren Schulen, sondern bieten auch die Möglichkeit eines aktiven und anschaulichen Erwerbens von Wissen und Kompetenzen. Mit Ausnahme der Mikroskope sind die Utensilien für die Einheiten kostengünstig und einfach zu beschaffen; die überwiegend angewandte Methode ist die „non-flooded petri dish“ Technik.  © Susanne Riedl