Membrane Biophysics


Pollen Systems BiologyHow do pollen grains germinate and generate a highly polarized pollen tube?
Although a detailed characterization of single ion transporters helps to understand their specific role during pollen tube growth, a comprehensive view of the entire proteins during germination and tube growth can provide new insights into the polar growth process. Therefore, after studying membrane and membrane-associated proteins in pollen by a membrane proteome analysis  (Pertl et al 2009), we identified the lily pollen transcriptome (Lang et al. 2015), which is now used as a customized database for further proteome studies.
In addition, specific protein-protein interactions (PM H+ ATPase interactome: Pertl-Obermeyer et al. 2014) are studied as well as metabolic pathways networks (Obermeyer et al. 2013) that dynamically change during germination and growth of pollen tubes. By using this systems biological (multi-omics) approach one may identify and map essential processes pathways and molecules that form the tip growth network = TIPOME.